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cd5-apc antibody  (Thermo Fisher)


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    Structured Review

    Thermo Fisher cd5-apc antibody
    Pseudotime analysis identifies regulators of differentiation, including AP-1 genes, that play a role in pre-leukemic B-1-cell identity. ( A ) B cell-specified common lymphoid progenitors (CLPs) progress through multiple stages of development to become immature B cells after undergoing V(D)J antigen receptor heavy (H) and light (L) chain recombination. B-2 cell development occurs in the bone marrow, whereas B-1 cell development occurs in the fetal liver. Early B-1 progenitor cells have been identified in the yolk sac as early as E8.25. Immature B-1 cells differentiate into <t>CD5</t> + B-1a or CD5- B-1b cells in the periphery, predominantly in serous cavities (e.g. pleural, peritoneal cavities). Expression of defining cell surface markers are indicated below each cell type. Cell surface markers expressed during B-1 cell development are not well defined. sIgM, surface immunoglobulin M. Created with BioRender.com. ( B ) B-1 (left) or B-2 (right) signature gene sets from Fitch et al. . Expression of signature genes in scRNA-seq data from Prdm14 -expressing pre-leukemic B cells is indicated, with significant differentially expressed genes highlighted in green (up-regulated) or orange (down-regulated). ( C ) Gene Ontology analysis of down-regulated genes in sorted pre-leukemia cells relative to immunophenotype-matched controls. ( D ) Pseudotime analysis was restricted to one manifold which included B cell-associated clusters 0, 1, 2, 11, and 17. One lineage was identified, beginning at cluster 11 and ending at cluster 1. ( E ) Thirty transcription factors were significantly differentially expressed along the pseudotime axis. ( F ) GO analysis of the 30 transcription factors identified enrichment of various biological processes.
    Cd5 Apc Antibody, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cd5-apc+antibody/pmc11909259-43-0-2?v=Thermo+Fisher
    Average 90 stars, based on 1 article reviews
    cd5-apc antibody - by Bioz Stars, 2026-07
    90/100 stars

    Images

    1) Product Images from "Functional and molecular single-cell analyses implicate PRDM14 in the initiation of B cell leukemia in mice"

    Article Title: Functional and molecular single-cell analyses implicate PRDM14 in the initiation of B cell leukemia in mice

    Journal: Scientific Reports

    doi: 10.1038/s41598-025-93043-z

    Pseudotime analysis identifies regulators of differentiation, including AP-1 genes, that play a role in pre-leukemic B-1-cell identity. ( A ) B cell-specified common lymphoid progenitors (CLPs) progress through multiple stages of development to become immature B cells after undergoing V(D)J antigen receptor heavy (H) and light (L) chain recombination. B-2 cell development occurs in the bone marrow, whereas B-1 cell development occurs in the fetal liver. Early B-1 progenitor cells have been identified in the yolk sac as early as E8.25. Immature B-1 cells differentiate into CD5 + B-1a or CD5- B-1b cells in the periphery, predominantly in serous cavities (e.g. pleural, peritoneal cavities). Expression of defining cell surface markers are indicated below each cell type. Cell surface markers expressed during B-1 cell development are not well defined. sIgM, surface immunoglobulin M. Created with BioRender.com. ( B ) B-1 (left) or B-2 (right) signature gene sets from Fitch et al. . Expression of signature genes in scRNA-seq data from Prdm14 -expressing pre-leukemic B cells is indicated, with significant differentially expressed genes highlighted in green (up-regulated) or orange (down-regulated). ( C ) Gene Ontology analysis of down-regulated genes in sorted pre-leukemia cells relative to immunophenotype-matched controls. ( D ) Pseudotime analysis was restricted to one manifold which included B cell-associated clusters 0, 1, 2, 11, and 17. One lineage was identified, beginning at cluster 11 and ending at cluster 1. ( E ) Thirty transcription factors were significantly differentially expressed along the pseudotime axis. ( F ) GO analysis of the 30 transcription factors identified enrichment of various biological processes.
    Figure Legend Snippet: Pseudotime analysis identifies regulators of differentiation, including AP-1 genes, that play a role in pre-leukemic B-1-cell identity. ( A ) B cell-specified common lymphoid progenitors (CLPs) progress through multiple stages of development to become immature B cells after undergoing V(D)J antigen receptor heavy (H) and light (L) chain recombination. B-2 cell development occurs in the bone marrow, whereas B-1 cell development occurs in the fetal liver. Early B-1 progenitor cells have been identified in the yolk sac as early as E8.25. Immature B-1 cells differentiate into CD5 + B-1a or CD5- B-1b cells in the periphery, predominantly in serous cavities (e.g. pleural, peritoneal cavities). Expression of defining cell surface markers are indicated below each cell type. Cell surface markers expressed during B-1 cell development are not well defined. sIgM, surface immunoglobulin M. Created with BioRender.com. ( B ) B-1 (left) or B-2 (right) signature gene sets from Fitch et al. . Expression of signature genes in scRNA-seq data from Prdm14 -expressing pre-leukemic B cells is indicated, with significant differentially expressed genes highlighted in green (up-regulated) or orange (down-regulated). ( C ) Gene Ontology analysis of down-regulated genes in sorted pre-leukemia cells relative to immunophenotype-matched controls. ( D ) Pseudotime analysis was restricted to one manifold which included B cell-associated clusters 0, 1, 2, 11, and 17. One lineage was identified, beginning at cluster 11 and ending at cluster 1. ( E ) Thirty transcription factors were significantly differentially expressed along the pseudotime axis. ( F ) GO analysis of the 30 transcription factors identified enrichment of various biological processes.

    Techniques Used: Expressing

    Antibodies used for the CyTOF experiments.
    Figure Legend Snippet: Antibodies used for the CyTOF experiments.

    Techniques Used:

    Antibodies used for the flow cytometry experiments.
    Figure Legend Snippet: Antibodies used for the flow cytometry experiments.

    Techniques Used: Flow Cytometry



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    Pseudotime analysis identifies regulators of differentiation, including AP-1 genes, that play a role in pre-leukemic B-1-cell identity. ( A ) B cell-specified common lymphoid progenitors (CLPs) progress through multiple stages of development to become immature B cells after undergoing V(D)J antigen receptor heavy (H) and light (L) chain recombination. B-2 cell development occurs in the bone marrow, whereas B-1 cell development occurs in the fetal liver. Early B-1 progenitor cells have been identified in the yolk sac as early as E8.25. Immature B-1 cells differentiate into <t>CD5</t> + B-1a or CD5- B-1b cells in the periphery, predominantly in serous cavities (e.g. pleural, peritoneal cavities). Expression of defining cell surface markers are indicated below each cell type. Cell surface markers expressed during B-1 cell development are not well defined. sIgM, surface immunoglobulin M. Created with BioRender.com. ( B ) B-1 (left) or B-2 (right) signature gene sets from Fitch et al. . Expression of signature genes in scRNA-seq data from Prdm14 -expressing pre-leukemic B cells is indicated, with significant differentially expressed genes highlighted in green (up-regulated) or orange (down-regulated). ( C ) Gene Ontology analysis of down-regulated genes in sorted pre-leukemia cells relative to immunophenotype-matched controls. ( D ) Pseudotime analysis was restricted to one manifold which included B cell-associated clusters 0, 1, 2, 11, and 17. One lineage was identified, beginning at cluster 11 and ending at cluster 1. ( E ) Thirty transcription factors were significantly differentially expressed along the pseudotime axis. ( F ) GO analysis of the 30 transcription factors identified enrichment of various biological processes.
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    Image Search Results


    Pseudotime analysis identifies regulators of differentiation, including AP-1 genes, that play a role in pre-leukemic B-1-cell identity. ( A ) B cell-specified common lymphoid progenitors (CLPs) progress through multiple stages of development to become immature B cells after undergoing V(D)J antigen receptor heavy (H) and light (L) chain recombination. B-2 cell development occurs in the bone marrow, whereas B-1 cell development occurs in the fetal liver. Early B-1 progenitor cells have been identified in the yolk sac as early as E8.25. Immature B-1 cells differentiate into CD5 + B-1a or CD5- B-1b cells in the periphery, predominantly in serous cavities (e.g. pleural, peritoneal cavities). Expression of defining cell surface markers are indicated below each cell type. Cell surface markers expressed during B-1 cell development are not well defined. sIgM, surface immunoglobulin M. Created with BioRender.com. ( B ) B-1 (left) or B-2 (right) signature gene sets from Fitch et al. . Expression of signature genes in scRNA-seq data from Prdm14 -expressing pre-leukemic B cells is indicated, with significant differentially expressed genes highlighted in green (up-regulated) or orange (down-regulated). ( C ) Gene Ontology analysis of down-regulated genes in sorted pre-leukemia cells relative to immunophenotype-matched controls. ( D ) Pseudotime analysis was restricted to one manifold which included B cell-associated clusters 0, 1, 2, 11, and 17. One lineage was identified, beginning at cluster 11 and ending at cluster 1. ( E ) Thirty transcription factors were significantly differentially expressed along the pseudotime axis. ( F ) GO analysis of the 30 transcription factors identified enrichment of various biological processes.

    Journal: Scientific Reports

    Article Title: Functional and molecular single-cell analyses implicate PRDM14 in the initiation of B cell leukemia in mice

    doi: 10.1038/s41598-025-93043-z

    Figure Lengend Snippet: Pseudotime analysis identifies regulators of differentiation, including AP-1 genes, that play a role in pre-leukemic B-1-cell identity. ( A ) B cell-specified common lymphoid progenitors (CLPs) progress through multiple stages of development to become immature B cells after undergoing V(D)J antigen receptor heavy (H) and light (L) chain recombination. B-2 cell development occurs in the bone marrow, whereas B-1 cell development occurs in the fetal liver. Early B-1 progenitor cells have been identified in the yolk sac as early as E8.25. Immature B-1 cells differentiate into CD5 + B-1a or CD5- B-1b cells in the periphery, predominantly in serous cavities (e.g. pleural, peritoneal cavities). Expression of defining cell surface markers are indicated below each cell type. Cell surface markers expressed during B-1 cell development are not well defined. sIgM, surface immunoglobulin M. Created with BioRender.com. ( B ) B-1 (left) or B-2 (right) signature gene sets from Fitch et al. . Expression of signature genes in scRNA-seq data from Prdm14 -expressing pre-leukemic B cells is indicated, with significant differentially expressed genes highlighted in green (up-regulated) or orange (down-regulated). ( C ) Gene Ontology analysis of down-regulated genes in sorted pre-leukemia cells relative to immunophenotype-matched controls. ( D ) Pseudotime analysis was restricted to one manifold which included B cell-associated clusters 0, 1, 2, 11, and 17. One lineage was identified, beginning at cluster 11 and ending at cluster 1. ( E ) Thirty transcription factors were significantly differentially expressed along the pseudotime axis. ( F ) GO analysis of the 30 transcription factors identified enrichment of various biological processes.

    Article Snippet: CD5-APC , eBioscience , 53 − 7.3.

    Techniques: Expressing

    Antibodies used for the CyTOF experiments.

    Journal: Scientific Reports

    Article Title: Functional and molecular single-cell analyses implicate PRDM14 in the initiation of B cell leukemia in mice

    doi: 10.1038/s41598-025-93043-z

    Figure Lengend Snippet: Antibodies used for the CyTOF experiments.

    Article Snippet: CD5-APC , eBioscience , 53 − 7.3.

    Techniques:

    Antibodies used for the flow cytometry experiments.

    Journal: Scientific Reports

    Article Title: Functional and molecular single-cell analyses implicate PRDM14 in the initiation of B cell leukemia in mice

    doi: 10.1038/s41598-025-93043-z

    Figure Lengend Snippet: Antibodies used for the flow cytometry experiments.

    Article Snippet: CD5-APC , eBioscience , 53 − 7.3.

    Techniques: Flow Cytometry